In vitro > 항암유효성평가지원센터

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In vitro

신규 항암물질의 in vitro 효능 평가를 통하여 개발 물질의 선별 및 최적화, 적용 암종 선정, 작용기전의 규명, 바이오마커 선정함으로써 다음 단계의 in vivo 동물 실험의 근거를 제시하는 가장 기본적인 시험단계

보유모델

암종

Human

Mouse

Bladder

J82, T24, UM-UC-3

Brain

SF268, SF295, SF539, SNB19, SNB5, U-251

Breast

BT-549, Hs 578T, MCF7, MCF10A, MDA-MB-231, MDA-MB-468, SK-BR-3, T47D

4T1, 4T1-Luc-TdTomato

Cervix

A431, CaSki, C33, HeLa, SiHa

Cholangiocarcinoma

SNU-1079, SNU-1196, SSP-25, TFK-1

Colon

Caco-2, COLO205, DLD-1, HCT-8, HCT-15, HCT-116, HT-29, KM12C, LoVo, LS1034, NCI-H716, NCI-H747, SNU-769B, SNU-1047, SNU-1235, SNU-C1, SNU-C2A, SNU-C2B, SNU-C5, SW403, SW480, SW620, RKO, WiDr

CT26, CT26-Luc, MC38, MC38-Luc

Esophagus

OE33

Fibrosarcoma

FSa-ll

Glioblastoma

GL26

Head and Neck

CAL27, SCC-9, SCC-15, SCC-25

Kidney

ACHN, A-498 Caki-1, RXF393, SN12C, SN12C-PM6, TK10, UO31, 786-O

Hematologic
Malignancy		 Myeloid MOLM-13, MOLM-14, MV-4-11, OCI-AML2, THP-1, U937, KG-1, SKM-1, BDCM, HL-60, NB4, K562, MEG-01, KU812, RPMI8226 WEHI-3
Lymphoid RCH-ACV, TOM-1, NALM-20, Kasumi-2, CCRF-CEM, Jurkat, MOLT-4, Daudi, Raji, SR Ba/F3

Liver

Hep3B, HepG2, HepG2-Luc-Fluc, Huh7, SK-HEP-1, SNU-182, SNU-398, SNU-449

Lung

A549, A549-Luc, Calu-1, Calu-3, EKVX, HCC15, HCC78, HCC95, HCC366, HCC827, HOP62, HOP92, LC-2/ad, LK-2, NCI-H23, NCI-H69, NCI-H82, NCI-H146, NCI-H187, NCI-H209, NCI-H226, NCI-H322M, NCI-H358, NCI-H441, NCI-H460, NCI-H520, NCI-H522, NCI-H526, NCI-H596, NCI-H647, NCI-H820, NCI-H1299, NCI-H1385, NCI-H1395, NCI-H1473, NCI-H1573, NCI-H1581, NCI-H1650, NCI-H1666, NCI-H1703, NCI-H1781, NCI-H1944, NCI-H1975, NCI-H2009, NCI-H2170, NCI-H2228, NCI-H2286, NCI-H3122, PC9

LLC1

LLC1-Luc

Melanoma

A375-P, LOX-IMVI, Malme-3M, M14, MDA-MB-435, SK-MEL-2, SKMEL-5, SKMEL-28, UACC-62, UACC-257

B16-F10

Ovary

A2780, ES-2-Luc, IGROV1, NCI/ADR-RES, OVCAR-3, OVCAR-3-Luc, OVCAR-4, OVCAR-5, OVCAR-8, SK-OV-3, SK-OV-3-Luc, SNU-8, SNU-251, UWB1.289 + BRCA1 null, UWB1.289 + BRCA1 overexpression

ID8

Pancreas

AsPC-1, AsPC-1-Luc, BxPC-3, BxPC-3-Luc, MIAPaCa-2, PANC-1

PAN 02

Prostate

DU 145, LNCaP, PC3, VCaP, 22Rv1

TRAMP-C1

Stomach

OE19, SNU-1, SNU-5, NCI-N87

서비스 내용

  • In vitro drug efficacy test : Cell viability / Death assay / IC50 calculation / Synergism analysis
  • In vitro tumorigenic potential test : Clonogenic assay
  • Signal pathway analysis : Immuno blotting, Immunoprecipitation, ELISA, Q-PCR, Chromatin Immunoprecipitation, Transfection, Protein binding assay
  • Enzyme assay : Kinase assay, Methyltransferase assay
  • Immunocytochemistry, TUNEL staining, Immunofluorescence staining, Hypoxia staining, Pimonidazole, ROS staining, Calcium/zinc ion staining, MitoTracker/sensor staining, LysoTracker/sensor staining, Autophagic vacuole staining
  • MOA 검증

서비스 예시 (IC50 calculation)

  • 세포주에서 Combination Index (CI)
    산출을 통한 병용효과 확인

  • Enzyme assay 및 IC50 산출

  • In vitro 세포 생존율 측정 및 IC50 산출